IkB-alpha [p Ser36] Antibody (SR1657) - BSA Free

Name: IkB-alpha [p Ser36] Antibody (SR1657) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-21594
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-21594

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1657 expressed in HEK293

Format

BSA Free

View all IkB-alpha Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-IkB-alpha [Ser36] (Uniprot #: P25963)

Modification

p Ser 36

Specificity

Detects endogenous levels of total phospho-IkB-alpha [Ser36]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IkB-alpha [p Ser36] Antibody (SR1657) - BSA Free

Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] -

Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] - Western blot analysis using NBP3-21594 in HeLa cell lysate treated with Calyculin A.

Applications for IkB-alpha [p Ser36] Antibody (SR1657) - BSA Free

Application

Recommended Usage

Flow Cytometry

1:50

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Ser36] Antibody (SR1657) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IkB-alpha [p Ser36] Antibody (SR1657) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.