IkB-alpha Antibody - BSA Free

Name: IkB-alpha Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP3-23384
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23384

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

IkB-alpha (C-terminus) synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human IkB-alpha. This peptide sequence has high homology to rat and mouse IkB-alpha, and low homology to other IkB proteins. [UniProt# P25963]

Specificity

This antibody detects a 38 kDa protein on SDS-PAGE immunoblots of human A431 and Jurkat cells, as well as mouse C2C12 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for IkB-alpha Antibody - BSA Free

Western Blot: IkB-alpha Antibody [NBP3-23384] -

Western Blot: IkB-alpha Antibody [NBP3-23384] - Western blot image of human A431. The Blots were probed with anti-IkB-alpha (C-term.) polyclonal antibody at a dilution of 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).

Applications for IkB-alpha Antibody - BSA Free

Application

Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS and 50% glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

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Product Specific Notices for IkB-alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.