IkB-alpha [p Tyr42] Antibody

Name: IkB-alpha [p Tyr42] Antibody
Brand: Novus Biologicals
SKU: NBP3-23389
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23389

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all IkB-alpha Primary Antibodies »

Product Specifications

Immunogen

IkB-alpha (Tyr-42) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 42 of human IkB-alpha. This peptide sequence has low homology to other I?B proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif. [UniProt# P25963]

Modification

p Tyr42

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IkB-alpha (Tyr-42) peptide (without carrier). The antibody detects a 38 kDa protein on SDS-PAGE immunoblots of A431 and Jurkat cells treated with pervanadate, but not in control cells. Due to homologies to tyrosine sites on other proteins, it is recommended that the antibody be used to detect phosphorylation of immunoprecipitated IkB-alpha.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for IkB-alpha [p Tyr42] Antibody

Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] -

Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).

Applications for IkB-alpha [p Tyr42] Antibody

Application

Recommended Usage

Immunoprecipitation

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Tyr42] Antibody

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Product Specific Notices for IkB-alpha [p Tyr42] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.