IL-1 alpha/IL-1F1 Antibody (OTI2F8)

Novus Biologicals | Catalog # NBP2-45400

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F8
View all IL-1 alpha/IL-1F1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IL1A(NP_000566) produced in HEK293T cell.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30213497). Mouse reactivity reported in scientific literature (PMID: 30240702). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-1 alpha/IL-1F1 Antibody (OTI2F8)

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)
Immunocytochemistry/ Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunocytochemistry/ Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunocytochemistry/Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400] - IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL-1 alpha.
Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL-1 alpha (NP_000566) produced in Analysis of HEK293T cell (5 ug, right lane).
Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400]

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for IL-1 alpha/IL-1F1 Antibody (OTI2F8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-1 alpha/IL-1F1

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Gene Symbol

IL1A

Additional IL-1 alpha/IL-1F1 Products

Product Documents for IL-1 alpha/IL-1F1 Antibody (OTI2F8)

Certificate of Analysis

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Product Specific Notices for IL-1 alpha/IL-1F1 Antibody (OTI2F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-1 alpha/IL-1F1 Antibody (OTI2F8)

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Protocols

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FAQs

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail