IL-10 Antibody (JES3-12G8) [FITC]

Novus Biologicals | Catalog # NBP2-27577F

Novus Biologicals

Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Virus

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, ELISA, ELISA Detection (Matched Antibody Pair)

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # JES3-12G8
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Product Specifications

Immunogen

COS-expressed human IL-10

Specificity

Human/Viral/Cynomolgus IL-10

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for IL-10 Antibody (JES3-12G8) [FITC]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-10

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for IL-10 Antibody (JES3-12G8) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IL-10 Antibody (JES3-12G8) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-10 Antibody (JES3-12G8) [FITC]

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  • Q: Does viral IL-10 cross-react in bioassay?

    A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

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