IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81040

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Neutralization, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 125-2H

Format

Azide and BSA Free
View all IL-18/IL-1F4 Primary Antibodies »

Product Specifications

Immunogen

Fusion of splenocytes from mice immunized with human IL-18/IL-1F4, with SP2/O mouse myeloma cells.

Specificity

Recognizes the active form of human IL-18/IL-1F4.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free

Western Blot: IL-18/IL-1F4 Antibody (125-2H)ChimericAzide and BSA Free [NBP2-81040]

Western Blot: IL-18/IL-1F4 Antibody (125-2H)ChimericAzide and BSA Free [NBP2-81040]

Western Blot: IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040] - Western Blot using IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040]. HeLa cell lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H [NBP2-81040] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. NBP2-81040 successfully detected IL18 in HeLa cell lysate samples.

Applications for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IL-18/IL-1F4

The protein encoded by the IL18 gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. (provided by RefSeq)

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-18/IL-1F4 Antibody (125-2H) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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