Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Enzyme-linked Immunosorbent Spot, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B-G5

Format

Azide and BSA Free
View all IL-2 Primary Antibodies »

Product Specifications

Immunogen

Natural human IL-2

Specificity

recognizes both natural and recombinant human IL-2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells

Scientific Data Images for IL-2 Antibody (B-G5) - Azide and BSA Free

Flow Cytometry: IL-2 Antibody (B-G5) - Azide and BSA Free [NBP3-14593]

Flow Cytometry: IL-2 Antibody (B-G5) - Azide and BSA Free [NBP3-14593]

Flow Cytometry: IL-2 Antibody (B-G5) [NBP3-14593] - The graph on the left represents activated lymphocytes marked with IL-2 antibody. The graph on the right shows IL-2 antibody with non-activated lymphocytes. In the two cases, the dotted line represents the IgG1 isotype control.

Applications for IL-2 Antibody (B-G5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Enzyme-linked Immunosorbent Spot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Biological Activity: Inhibits IL-2 induced proliferation on Kit 225 cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS. Sterile-filtered through 0.22 um.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IL-2

Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL2 may be of use as an anti-tumor agent in cancer therapy. Recombinant human IL2 is a 15.5 kDa protein, consisting of 134 amino acid residues. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for IL-2 Antibody (B-G5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-2 Antibody (B-G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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