IL-21 Antibody - BSA Free

Name: IL-21 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-76740
Price: 199 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-76740

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Independent Antibodies, Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, ELISA, Block/Neutralize

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all IL-21 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to 15 amino acids near the center of human IL-21 precursor. The immunogen is located within the last 50 amino acids of IL-21. Amino Acid Squence: TCPSCDSYEKKPPKE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Pig: (87%)

Specificity

At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-21 Antibody - BSA Free

Western Blot: IL-21 AntibodyBSA Free [NBP1-76740]

Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: IL-21 Antibody - BSA Free [NBP1-76740]

Immunocytochemistry/Immunofluorescence: IL-21 Antibody [NBP1-76740] - Immunocytochemistry Validation of IL-21 in K562 Cells.Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (NBP1-76740) at 2 ug/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: IL-21 AntibodyBSA Free [NBP1-76740]

Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: IL-21 AntibodyBSA Free [NBP1-76740]

Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740 (1 ug/mL), IL-21 from Company B (5 ug/mL), beta-actin (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] -

Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell Lysate
Loading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.

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Applications for IL-21 Antibody - BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/mL

Application Notes

Use in B/N reported in scientific literature (PMID:34408754).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-21

A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Gene Symbol

IL21

UniProt

NP_068575 (Human)

Additional IL-21 Products

Product Documents for IL-21 Antibody - BSA Free

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Product Specific Notices for IL-21 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.