IL-33 Antibody (CL13438) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43843

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13438

Format

Azide and BSA Free
View all IL-33 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O95760, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for IL-33 Antibody (CL13438) - Azide and BSA Free

IL-33 Antibody (CL13438) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: IL-33 Antibody [NBP3-43843]

Immunocytochemistry/ Immunofluorescence: IL-33 Antibody [NBP3-43843]

Staining in EFO-21 cell line with Anti-IL-33 monoclonal antibody).
IL-33 Antibody (CL13438) - Azide and BSA Free Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Analysis in human urinary bladder and skeletal muscle tissues using NBP3-43843 antibody. Corresponding IL-33 RNA-seq data are presented for the same tissues.
IL-33 Antibody (CL13438) - Azide and BSA Free Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
IL-33 Antibody (CL13438) - Azide and BSA Free Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
IL-33 Antibody (CL13438) - Azide and BSA Free Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
IL-33 Antibody (CL13438) - Azide and BSA Free Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Immunohistochemistry-Paraffin: IL-33 Antibody [NBP3-43843]

Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Applications for IL-33 Antibody (CL13438) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-33

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Gene Symbol

IL33

Additional IL-33 Products

Product Documents for IL-33 Antibody (CL13438) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-33 Antibody (CL13438) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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