IL-7R alpha/CD127 Antibody (BLR177J)

Name: IL-7R alpha/CD127 Antibody (BLR177J)
Brand: Novus Biologicals
SKU: NBP3-14749
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14749

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR177J

View all IL-7R alpha/CD127 Primary Antibodies »

Product Specifications

Immunogen

residues 21-236 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for IL-7R alpha/CD127 Antibody (BLR177J)

Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]

Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.

Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]

Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]

Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] -

Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]- Rabbit anti-CD127/IL-7R alpha recombinantmonoclonal antibody [BLR177J] Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.

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Applications for IL-7R alpha/CD127 Antibody (BLR177J)

Application

Recommended Usage

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

6 µl/1 mg lysate

Western Blot

1:1000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with, BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: IL-7R alpha/CD127

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Gene Symbol

IL7R

Additional IL-7R alpha/CD127 Products

Product Documents for IL-7R alpha/CD127 Antibody (BLR177J)

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Certificate of Analysis

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Product Specific Notices for IL-7R alpha/CD127 Antibody (BLR177J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.