IL-9 Antibody (RM9A4) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-12023

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RM9A4

Format

Azide and BSA Free
View all IL-9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising rats with recombinant full-length murine IL-9.

Specificity

This antibody is specific for murine IL-9.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL-9 Antibody (RM9A4) - Chimeric - Azide and BSA Free

Flow Cytometry: IL-9 Antibody (RM9A4) - Chimeric - Azide and BSA Free [NBP3-12023]

Flow Cytometry: IL-9 Antibody (RM9A4) - Chimeric - Azide and BSA Free [NBP3-12023]

Flow Cytometry: IL-9 Antibody (RM9A4) - Chimeric [NBP3-12023] - Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RM9A4 (NBP3-12023, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-12023. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Applications for IL-9 Antibody (RM9A4) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IL-9

IL-9 is a cytokine with immunoregulatory function, produced by activated T-helper type 2 lymphocytes. It acts on various cell types such as T- and B-lymphocytes, mast cells, haemopoietic progenitors and megakaryoblastic leukemia cell lines. IL-9 stimulates cell proliferation and prevents apoptosis. IL-9 may play a role in the pathogenesis of diseases such as certain lymphomas (Hodgin's disease, anaplastic lymphoma) and asthma. Although murine IL-9 can act on human cells, human IL-9 is inactive on mouse cells.

Long Name

Interleukin 9

Alternate Names

IL9

Gene Symbol

IL9

Additional IL-9 Products

Product Documents for IL-9 Antibody (RM9A4) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-9 Antibody (RM9A4) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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