Insulin Antibody (E2-E3 (same as INS04))

Novus Biologicals | Catalog # NBP2-34260

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Bovine, Rabbit

Cited:

Human, Mouse, Porcine, Bovine, Ferret, Primate - Macaca mulatta (Rhesus Macaque), Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # E2-E3 (same as INS04)
View all Insulin Primary Antibodies »

Product Specifications

Immunogen

Purified pig insulin, conjugated to KLH (Uniprot: P01308)

Localization

Cytoplasmic

Marker

beta-Cell & Insulinoma Marker

Specificity

Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34611)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Insulin Antibody (E2-E3 (same as INS04))

Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Insulin-Antibody-E2-E3-same-as-INS04-Western-Blot-NBP2-34260-img0006.jpg
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Insulin-Antibody-E2-E3-same-as-INS04-Immunohistochemistry-NBP2-34260-img0005.jpg
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.

Applications for Insulin Antibody (E2-E3 (same as INS04))

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 5 reviews rated 4.8 using NBP2-34260 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

UniProt

P01308

Additional Insulin Products

Product Documents for Insulin Antibody (E2-E3 (same as INS04))

Certificate of Analysis

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Product Specific Notices for Insulin Antibody (E2-E3 (same as INS04))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Insulin Antibody (E2-E3 (same as INS04))

Customer Reviews for Insulin Antibody (E2-E3 (same as INS04)) (5)

4.8 out of 5
5 Customer Ratings
5 Stars
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4 Stars
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Insulin Antibody (E2-E3 (same as INS04))
    Name: Anonymous
    Application: Immunofluorescence - paraffin
    Sample Tested: Adult pancreas
    Species: Human
    Verified Customer | Posted 01/20/2019
    Insulin E2E3, human pancreas (FFPE), HIER pH9
    pH 9 antigen retrieval
    Insulin Antibody (E2-E3 (same as INS04)) NBP2-34260
  • Insulin Antibody (E2-E3 (same as INS04))
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pancrea cells
    Species: Human
    Verified Customer | Posted 10/24/2017
    Insulin Antibody (E2-E3 (same as INS04)) NBP2-34260
  • Insulin Antibody (E2-E3 (same as INS04))
    Name: Ajay Ashok
    Application: Western Blot
    Sample Tested: Mouse Pancreas
    Species: Mouse
    Verified Customer | Posted 06/13/2017
    Works very well at a 1:1000 with sample protein of 40ug
  • Insulin Antibody (E2-E3 (same as INS04))
    Name: Ajay Ashok
    Application: Immunocytochemistry
    Sample Tested: mouse retina
    Species: Mouse
    Verified Customer | Posted 06/07/2017
    Insulin Antibody (E2-E3 (same as INS04)) NBP2-34260
  • Insulin Antibody (E2-E3 (same as INS04))
    Name: Ajay Ashok
    Application: Western Blot
    Sample Tested: Brain
    Species: Human
    Verified Customer | Posted 06/07/2017
    Insulin Antibody (E2-E3 (same as INS04)) NBP2-34260

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Protocols

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FAQs for Insulin Antibody (E2-E3 (same as INS04))

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  • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.

    A: I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

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