Insulin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87485

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Insulin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Insulin Antibody - BSA Free

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining in human pancreas and liver tissues using anti-INS antibody. Corresponding INS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485]

Immunohistochemistry-Paraffin: Insulin Antibody [NBP1-87485] - Staining of human pancreas shows high expression.

Applications for Insulin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Entrez Gene IDs

397415 (Porcine)

Gene Symbol

INS

Additional Insulin Products

Product Documents for Insulin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Insulin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Insulin Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Insulin Antibody - BSA Free

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  • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.

    A:

    I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

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