Integrin alpha 2b beta 3 Antibody (10E5) [CoraFluor™ 1]

Novus Biologicals | Catalog # NBP2-81080CL1

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 10E5
View all Integrin alpha 2b beta 3 Primary Antibodies »

Product Specifications

Immunogen

Human blood platelets.

Specificity

The antibody binds to Integrin alpha 2b beta 3 with an affinity (Kd) of 16.2+-6 nM. The antibody completely inhibits platelet aggregation at 7.8 ug/ml.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for Integrin alpha 2b beta 3 Antibody (10E5) [CoraFluor™ 1]

Integrin alpha 2b beta 3 Antibody (10E5) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Integrin alpha 2b beta 3 Antibody (10E5) [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Integrin alpha 2b beta 3

Integrin alpha 2b beta 3, also known as GPIIbIIIa, is the most abundant Integrin expressed by megakaryocytes and platelets, both on the surface and within alpha granules. Deficiencies of alpha 2b beta 3 produce Glanzmann thrombasthenia, a potentially serious bleeding disorder. Active cell surface alpha 2b beta 3 adheres to Fibrinogen, mediating platelet/platelet interactions that initiate a cascade of platelet activation and aggregation, extracellular matrix adhesion, formation of thrombi, and clot retraction. It also binds matrix proteins that have an RGD motif, including Fibronectin, Plasminogen, Prothrombin, Thrombospondin, and Vitronectin.

 

Alternate Names

BDPLT16;BDPLT2;CD41;CD41B;GP2B;GPIIb;GT;GT1;GTA;HPA3;Integrin alpha-IIb;ITGA2B;PPP1R93

Gene Symbol

ITGA2B

Additional Integrin alpha 2b beta 3 Products

Product Documents for Integrin alpha 2b beta 3 Antibody (10E5) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Integrin alpha 2b beta 3 Antibody (10E5) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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