Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-52708

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Citations (2)

Product Documents

Recombinant Monoclonal Antibody. Clone K20 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, CyTOF-ready

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # K20

Format

Azide and BSA Free

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Product Specifications

Immunogen

Human T-cell lymphoma cells injected into Biozzi high-responder mice.

Specificity

Recognises human CD29.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free (NBP2-52708) is a recombinant monoclonal antibody validated for use in IHC, Flow and IP. Anti-Integrin beta 1/CD29 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free [NBP2-52708]

Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) [NBP2-52708] - Flow-cytometry using anti-CD29 antibody K20 (NBP2-52708) Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of K20 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry-Frozen

1:10 - 1:500

Application Notes

This antibody is Cytof ready.

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

Download Product Datasheets

Product Specific Notices for Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules

Adhesion Molecules on Endothelial Cells

Adipose-derived Stem Cell Markers

Cancer Biomarkers

Cardiac Stem Cell Markers

Epithelial Cell Polarity

Integrins and Associated Molecules

Mesenchymal Stem Cell Markers

Skin Stem Cell Markers

Citations for Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

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Protocols

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FAQs for Integrin beta 1/CD29 Antibody (K20) - Azide and BSA Free

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Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.