Integrin beta 3/CD61 Antibody (SJ19-09)

Novus Biologicals | Catalog # NBP2-67416

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SJ19-09 expressed in HEK293
View all Integrin beta 3/CD61 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Integrin beta 3/CD61 aa 729-788 / 788. (SwissProt: P05106 Human; SwissProt: O54890 Mouse; Entrez Gene: 29302 Rat)

Localization

Cell membrane, Cell projection, Cell junction.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin beta 3/CD61 Antibody (SJ19-09)

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: mouse marrow tissue lysate
Immunocytochemistry/ Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunocytochemistry/ Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse lung tissue. IF-stained paraffin section. Image from verified customer review.
Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse bone marrow cell lysate with DMSO (lane 1), chemical agonist #1 (lane 2), chemical agonist #2 (lane 3), chemical agonist #3 (lane 4). WB image submitted by a verfied customer review.
Immunocytochemistry/ Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunocytochemistry/ Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human spleen tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse testis tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Integrin beta 3/CD61 Antibody (SJ19-09) Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416]

Western blot analysis of U-87 MG cell lysates with NBP2-67416 at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67416 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Integrin beta 3/CD61 Antibody (SJ19-09)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:400

Western Blot

1:1000

Reviewed Applications

Read 2 reviews rated 5 using NBP2-67416 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 3/CD61

Platelet membrane glycoprotein Integrin beta-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3). be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).

Alternate Names

CD61, GP3A, GPIIIA, INGRB3, ITGB3

Gene Symbol

ITGB3

Additional Integrin beta 3/CD61 Products

Product Documents for Integrin beta 3/CD61 Antibody (SJ19-09)

Certificate of Analysis

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Product Specific Notices for Integrin beta 3/CD61 Antibody (SJ19-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin beta 3/CD61 Antibody (SJ19-09)

Customer Reviews for Integrin beta 3/CD61 Antibody (SJ19-09) (2)

5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Integrin beta 3/CD61 Antibody (SJ19-09)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse primary cell and macrophage cell lysate
    Species: Mouse
    Verified Customer | Posted 09/14/2019
    Mouse bone marrow cells. Lane 1: DMSO; Lane 2: chemical agonist #1; Lane 3: chemical agonist #2; Lane 4: chemical agonist #3.
    Integrin beta 3/CD61 Antibody (SJ19-09) NBP2-67416
  • Integrin beta 3/CD61 Antibody (SJ19-09)
    Name: Anonymous
    Application: immunofluorescence staining
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 07/05/2019
    Paraffin Section
    Integrin beta 3/CD61 Antibody (SJ19-09) NBP2-67416

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