IRAK1 Antibody (3F7) - Azide and BSA Free

Novus Biologicals | Catalog # H00003654-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F7

Format

Azide and BSA Free
View all IRAK1 Primary Antibodies »

Product Specifications

Immunogen

IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS

Localization

interleukin-1 receptor complex

Specificity

IRAK1 - interleukin-1 receptor-associated kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IRAK1 Antibody (3F7) - Azide and BSA Free

Western Blot: IRAK1 Antibody (3F7) [H00003654-M01]

Western Blot: IRAK1 Antibody (3F7) [H00003654-M01]

Western Blot: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M01), clone 3F7.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody (3F7) [H00003654-M01]

Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody (3F7) [H00003654-M01]

Immunocytochemistry/Immunofluorescence: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: IRAK1 Antibody (3F7) [H00003654-M01]

Immunohistochemistry-Paraffin: IRAK1 Antibody (3F7) [H00003654-M01]

Immunohistochemistry-Paraffin: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: IRAK1 Antibody (3F7) [H00003654-M01]

Western Blot: IRAK1 Antibody (3F7) [H00003654-M01]

Western Blot: IRAK1 Antibody (3F7) [H00003654-M01] - Western Blot analysis of IRAK1 expression in K-562 ( Cat # L009V1 ).
Immunoprecipitation: IRAK1 Antibody (3F7) [H00003654-M01]

Immunoprecipitation: IRAK1 Antibody (3F7) [H00003654-M01]

Immunoprecipitation: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: IRAK1 Antibody (3F7) [H00003654-M01] - Detection limit for recombinant GST tagged IRAK1 is approximately 0.1ng/ml as a capture antibody.

Applications for IRAK1 Antibody (3F7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRAK1

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Long Name

Interleukin-1 Receptor-Associated Kinase 1

Alternate Names

Il1rak, Pelle, Plpk

Entrez Gene IDs

3654 (Human)

Gene Symbol

IRAK1

OMIM

300283 (Human)

UniProt

AAH54000

Additional IRAK1 Products

Product Documents for IRAK1 Antibody (3F7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IRAK1 Antibody (3F7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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