IRAK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77068

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IRAK1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK. Amino Acid Squence: DRQGPEESDEFQS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%)

Specificity

At least four isoforms of IRAK1 are known to exist; this antibody will detect all four isoforms. IRAK1 antibody is predicted to not cross-react with other members of the IRAK protein family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRAK1 Antibody - BSA Free

Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 Antibody [NBP1-77068] - Species Activity in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL,), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 Antibody [NBP1-77068] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), IRAK from Company B (2 ug/mL), beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 AntibodyBSA Free [NBP1-77068]

Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IRAK recombinant protein per lane.Antibodies: IRAK NBP1-77068 (1: 1ug/mL, 2: 2 ug/mL and 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody - BSA Free [NBP1-77068]

Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody - BSA Free [NBP1-77068]

Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunocytochemistry Validation of IRAK in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-IRAK antibody (NBP1-77068) at 10 ug g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
IRAK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody - BSA Free [NBP1-77068] -

Immunocytochemistry/ Immunofluorescence: IRAK1 Antibody - BSA Free [NBP1-77068] - Figure 5 Immunofluorescence Validation of IRAK1 in Human HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for IRAK1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Immunoprecipitation

1:10-1:500

Western Blot

1-4 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRAK1

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

Long Name

Interleukin-1 Receptor-Associated Kinase 1

Alternate Names

Il1rak, Pelle, Plpk

Gene Symbol

IRAK1

UniProt

P51617 (Human)

Additional IRAK1 Products

Product Documents for IRAK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IRAK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IRAK1 Antibody - BSA Free

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Protocols

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