Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IRAK2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a. The immunogen is located within the last 50 amino acids of IRAK2. Amino Acid Squence: EAKRRLMENIVLYKEER

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%), Bovine (82%).

Specificity

At least four isoforms of IRAK2 are known to exist; this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit IRAK2 Antibody - BSA Free (NBP1-76641) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IRAK2 Antibody - BSA Free

Western Blot: IRAK2 AntibodyBSA Free [NBP1-76641]

Western Blot: IRAK2 AntibodyBSA Free [NBP1-76641]

Western Blot: IRAK2 Antibody [NBP1-76641] - RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
IRAK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRAK2 Antibody - BSA Free [NBP1-76641] -

Immunocytochemistry/ Immunofluorescence: IRAK2 Antibody - BSA Free [NBP1-76641] - Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 ug/mL.
IRAK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRAK2 Antibody - BSA Free [NBP1-76641] -

Immunocytochemistry/ Immunofluorescence: IRAK2 Antibody - BSA Free [NBP1-76641] - Immunofluorescence of IRAK2 in Hela cells with IRAK 2 antibody at 20 ug/mL.

Red: IRAK2 Antibody
Blue: DAPI staining
IRAK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRAK2 Antibody - BSA Free [NBP1-76641] -

Immunocytochemistry/ Immunofluorescence: IRAK2 Antibody - BSA Free [NBP1-76641] - Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 ug/mL.

Applications for IRAK2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1 - 10ug/ml

Western Blot

0.5 - 2ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRAK2

The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappaB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-'B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.

Long Name

Interleukin-1 Receptor-Associated Kinase 2

Alternate Names

interleukin-1 receptor associated kinase-2, interleukin-1 receptor-associated kinase 2, interleukin-1 receptor-associated kinase-like 2, IRAK-2, MGC150550

Gene Symbol

IRAK2

UniProt

AAO24761 (Mouse)

Additional IRAK2 Products

Product Documents for IRAK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IRAK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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