Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Polyclonal Rabbit IgG
View all IRAK2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a. The immunogen is located within the last 50 amino acids of IRAK2. Amino Acid Squence: EAKRRLMENIVLYKEER

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%), Bovine (82%).

Specificity

At least four isoforms of IRAK2 are known to exist; this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for IRAK2 Antibody [DyLight 550]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IRAK2

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

Long Name

Interleukin-1 Receptor-Associated Kinase 2

Alternate Names

interleukin-1 receptor associated kinase-2, interleukin-1 receptor-associated kinase 2, interleukin-1 receptor-associated kinase-like 2, IRAK-2, MGC150550

Gene Symbol

IRAK2

Additional IRAK2 Products

Product Documents for IRAK2 Antibody [DyLight 550]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IRAK2 Antibody [DyLight 550]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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