Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IRAK3 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M. The immunogen is located within the last 50 amino acids of IRAK-M. Amino Acid Squence: CSSKFSWDEYEQYKKE

Specificity

IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit IRAK3 Antibody - BSA Free (NBP1-76782) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IRAK3 Antibody - BSA Free

Western Blot: IRAK3 AntibodyBSA Free [NBP1-76782]

Western Blot: IRAK3 AntibodyBSA Free [NBP1-76782]

Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: IRAK3 Antibody - BSA Free [NBP1-76782]

Immunohistochemistry-Paraffin: IRAK3 Antibody - BSA Free [NBP1-76782]

Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-76782] - Staining of human spleen tissue with antibody at 5 ug/mL.
IRAK3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody - BSA Free [NBP1-76782] -

Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 ug/mL.

Red: IRAK3 Antibody
Blue: DAPI staining

Immunohistochemistry: IRAK3 Antibody [NBP1-76782] -

Immunohistochemistry: IRAK3 Antibody [NBP1-76782] - Rat Liver tissue with IRAK-M antibody at 10 ug/mL.
IRAK3 Antibody - BSA Free

Immunohistochemistry: IRAK3 Antibody - BSA Free [NBP1-76782] -

Immunohistochemistry: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunohistochemical staining of rat liver tissue using IRAK3 antibody at 2 ug/mL.

Applications for IRAK3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRAK3

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

Long Name

Interleukin-1 Receptor-Associated Kinase 3

Alternate Names

ASRT5, IRAK-M

Gene Symbol

IRAK3

UniProt

AF113136 (Human)

Additional IRAK3 Products

Product Documents for IRAK3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IRAK3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IRAK3 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IRAK3 Antibody - BSA Free

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    • A: Unfortunately, we cannot share the exact 16 amino acids of the peptide that the antibody was raised against because that info is proprietary. However, we can confirm you that the immunogen is located within the last 50 amino acids of IRAK-M protein ( C terminal or last 50 amino acids: P SQDLRPYKVN IDPSSEAPGH SCRSRPVESS CSSKFSWDEY EQYKKE; Uniprot ID Q9Y616). 
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