IRAK4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77231

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IRAK4 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK-4. The immunogen is located within the last 50 amino acids of IRAK-4. Amino Acid Squence: KKVQQLLQEMTAS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (85%), Mouse: (85%)

Specificity

IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRAK4 Antibody - BSA Free

Western Blot: IRAK4 AntibodyBSA Free [NBP1-77231]

Western Blot: IRAK4 AntibodyBSA Free [NBP1-77231]

Western Blot: IRAK4 Antibody [NBP1-77231] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IRAK4-NBP1-77231 (1 ug/mL), IRAK4 (1 ug/mL), beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody - BSA Free [NBP1-77231]

Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody - BSA Free [NBP1-77231]

Immunocytochemistry/Immunofluorescence: IRAK4 Antibody [NBP1-77231] - Immunocytochemical staining of IRAK-4 protein in K562 cells using IRAK4 antibody at 10 ug/ml concentration and HRP conjugated secondary - DAB chromogen detection method.
Western Blot: IRAK4 AntibodyBSA Free [NBP1-77231]

Western Blot: IRAK4 AntibodyBSA Free [NBP1-77231]

Western Blot: IRAK4 Antibody [NBP1-77231] - WB detection of IRAK4 protein in HeLa whole cell lysate using IRAK4 antibody using 1:1000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions respectively.
Western Blot: IRAK4 AntibodyBSA Free [NBP1-77231]

Western Blot: IRAK4 AntibodyBSA Free [NBP1-77231]

Western Blot: IRAK4 Antibody [NBP1-77231] - Western Blot Validation in Human Cell Lines. Loading: 15 ug/ of lysates per lane. Antibodies: IRAK4 NBP1-77231 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IRAK4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody - BSA Free [NBP1-77231] -

Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody - BSA Free [NBP1-77231] - Validation of IRAK4 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling IRAK4 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for IRAK4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRAK4

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Gene Symbol

IRAK4

UniProt

AAM15772 (Human)

Additional IRAK4 Products

Product Documents for IRAK4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IRAK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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