IRF4 Antibody (2D1) - Azide and BSA Free

Novus Biologicals | Catalog # H00003662-M05

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2D1

Format

Azide and BSA Free
View all IRF4 Primary Antibodies »

Product Specifications

Immunogen

IRF4 (NP_002451, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

Specificity

IRF4 (2D1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IRF4 Antibody (2D1) - Azide and BSA Free

Western Blot: IRF4 Antibody (2D1) [H00003662-M05]

Western Blot: IRF4 Antibody (2D1) [H00003662-M05]

Western Blot: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M05), clone 2D1. Lane 1: IRF4 transfected lysatE (51.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: IRF4 Antibody (2D1) [H00003662-M05]

Immunocytochemistry/ Immunofluorescence: IRF4 Antibody (2D1) [H00003662-M05]

Immunocytochemistry/Immunofluorescence: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of monoclonal antibody to IRF4 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: IRF4 Antibody (2D1) [H00003662-M05]

Western Blot: IRF4 Antibody (2D1) [H00003662-M05]

Western Blot: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of IRF4 over-expressed 293 cell line, cotransfected with IRF4 Validated Chimera RNAi or non-transfected control. Blot probed with H00003662-M05. GAPDH (36.1 kDa) used as loading control.
Sandwich ELISA: IRF4 Antibody (2D1) [H00003662-M05] - Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.

Applications for IRF4 Antibody (2D1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRF4

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells

Long Name

Interferon Regulatory Factor 4

Alternate Names

LSIRF, MUM1, NF-EM5

Entrez Gene IDs

3662 (Human)

Gene Symbol

IRF4

OMIM

601900 (Human)

UniProt

NP_002451 (Human)

Additional IRF4 Products

Product Documents for IRF4 Antibody (2D1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IRF4 Antibody (2D1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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