Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IRF7 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF7. The immunogen is located within amino acids 420-470 of IRF7. Amino Acid Squence: GQDLSAGRPKEKSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IRF7 Antibody - BSA Free

Western Blot: IRF7 AntibodyBSA Free [NBP1-77263]

Western Blot: IRF7 AntibodyBSA Free [NBP1-77263]

Western Blot: IRF7 Antibody [NBP1-77263] - 293 whole cell lysate with IRF7 antibody at (A) 0.5 and (B) 1 ug/ml and (C) 2 ug/ml.
IRF7 Antibody

Immunocytochemistry/Immunofluorescence: IRF7 Antibody [NBP1-77263] -

Immunocytochemistry/Immunofluorescence: IRF7 Antibody [NBP1-77263] - N9 cells stained for IRF-7 (Dilution 1:200). Image from verified customer review.
IRF7 Antibody - BSA Free

Immunohistochemistry: IRF7 Antibody - BSA Free [NBP1-77263] -

Immunohistochemistry: IRF7 Antibody - BSA Free [NBP1-77263] - Immunohistochemistry of IRF7 in human spleen tissue with IRF7 Antibody at 5 ug/mL.
IRF7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP1-77263] -

Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP1-77263] - Immunofluorescence of IRF7 in Mouse Spleen cells with IRF7 antibody at 20 ug/mL.
IRF7 Antibody - BSA Free

Immunohistochemistry: IRF7 Antibody - BSA Free [NBP1-77263] -

Immunohistochemistry: IRF7 Antibody - BSA Free [NBP1-77263] - Immunohistochemistry of IRF7 in mouse spleen tissue with IRF7 antibody at 5 ug/mL.

Applications for IRF7 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-1 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-77263 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF7

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

Alternate Names

interferon regulatory factor 7, interferon regulatory factor-7H, IRF-7, IRF7A, IRF-7H

Entrez Gene IDs

3665 (Human)

Gene Symbol

IRF7

UniProt

Q92985 (Human)

Additional IRF7 Products

Product Documents for IRF7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IRF7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IRF7 Antibody - BSA Free

Customer Reviews for IRF7 Antibody - BSA Free (1)

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  • IRF7 Antibody - BSA Free
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: N9 cell line
    Species: Mouse
    Verified Customer | Posted 04/07/2023
    N9 cells stained for IRF-7 (Dilution 1:200)
    IRF7 Antibody - BSA Free NBP1-77263

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Protocols

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FAQs

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