Islet-1 Antibody (1G9Q8)

Novus Biologicals | Catalog # NBP3-15315

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1G9Q8 expressed in HEK293
View all Islet-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Islet-1 (P61371). YAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Islet-1 Antibody (1G9Q8)

Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315]

Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315]

Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Western blot analysis of extracts of various cell lines, using Islet-1 Rabbit mAb (NBP3-15315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: Islet-1 Antibody (1G9Q8) [NBP3-15315]

Immunohistochemistry-Paraffin: Islet-1 Antibody (1G9Q8) [NBP3-15315]

Immunohistochemistry-Paraffin: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Immunohistochemistry of paraffin-embedded mouse fetal Heart using Islet-1 Rabbit mAb (NBP3-15315) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for Islet-1 Antibody (1G9Q8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Islet-1

Islet 1 encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Alternate Names

ISL1, Islet1

Gene Symbol

ISL1

Additional Islet-1 Products

Product Documents for Islet-1 Antibody (1G9Q8)

Certificate of Analysis

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Product Specific Notices for Islet-1 Antibody (1G9Q8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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