Isocitrate Dehydrogenase 1/IDH1 Antibody (CL0219) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43836

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0219

Format

Azide and BSA Free
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O75874, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images

Isocitrate Dehydrogenase 1/IDH1 Antibody (CL0219) - Azide and BSA Free Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Lane 1: Marker [kDa]
Lane 2: Human cell line RT-4
Isocitrate Dehydrogenase 1/IDH1 Antibody (CL0219) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Staining of A-431 cells using the anti-Isocitrate Dehydrogenase 1/IDH1 monoclonal antibody).
Isocitrate Dehydrogenase 1/IDH1 Antibody (CL0219) - Azide and BSA Free Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Staining of human kidney shows strong positivity in a subset of renal tubules.
Isocitrate Dehydrogenase 1/IDH1 Antibody (CL0219) - Azide and BSA Free Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Staining of human prostate shows strong positivity in glandular epithelium.
Isocitrate Dehydrogenase 1/IDH1 Antibody (CL0219) - Azide and BSA Free Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Staining of human testis shows strong immunoreactivity in seminiferous tubules.
Isocitrate Dehydrogenase 1/IDH1 Antibody (CL0219) - Azide and BSA Free Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP3-43836]

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Isocitrate Dehydrogenase 1/IDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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