Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70988

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2H9

Format

Azide and BSA Free
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human IDH1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)Azide and BSA Free [NBP2-70988]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)Azide and BSA Free [NBP2-70988]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Isocitrate dehydrogenase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant
Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Isocitrate dehydrogenase.
Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988] - Staining of paraffin-embedded endometrium tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)Azide and BSA Free [NBP2-70988]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)Azide and BSA Free [NBP2-70988]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Isocitrate dehydrogenase monoclonal antibody.
Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988] - Endometrium tissue.
Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Immunohistochemistry: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988] - Staining of paraffin-embedded breast tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.
Knockout Validated: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) - Azide and BSA Free [NBP2-70988] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IDH1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2,000 of Mouse Anti-Human IDH1 monoclonal Antibody (Catalog # NBP1-47804) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for IHD1 at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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