Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

Novus Biologicals | Catalog # NBP1-47804

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2H9
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human IDH1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

Knockout Validated: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IDH1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2,000 of Mouse Anti-Human IDH1 monoclonal Antibody (Catalog # NBP1-47804) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for IHD1 at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Isocitrate dehydrogenasemonoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804] - Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Isocitrate dehydrogenase.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Endometrium tissue.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Isocitrate dehydrogenase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Isocitrate dehydrogenase.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of paraffin-embedded breast tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of paraffin-embedded endometrium tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.
Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)[NBP1-47804]

Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)[NBP1-47804]Simple Western� analysis of endogenous protein PP5 (PPP5C) from SH-SY5Y lysates (0.2mg/mL) using PPP5C Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 4 using NBP1-47804 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Entrez Gene IDs

3417 (Human)

Gene Symbol

IDH1

UniProt

O75874

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9)

Customer Reviews for Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) (1)

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  • Name: Hongxia Hu
    Application: Western Blot
    Sample Tested: Hep3B WCL
    Species: Human
    Verified Customer | Posted 09/12/2012
    Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) NBP1-47804

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