Chemical Name: 4-[1-Hydroxy-2-[(1-methylethyl)amino]ethyl]-1,2-benzenediol hydrochloride
Biological ActivityIsoproterenol hydrochloride is a standard selective β-adrenoceptor agonist; vasorelaxant and bronchodilator. Activation of β2 receptors activates downstream PKA and ERK, and may stimulate NO-mediated endothelium-dependent smooth muscle relaxation. Active in vivo.
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Tocris products are intended for laboratory research use only, unless stated otherwise.
Nitric oxide (NO) primarily accounts for endothelium-dependent component of β-adrenoceptor-activated smooth muscle relaxation of mouse aorta in response to isoprenaline.
Akimoto et al.
J.Smooth Muscle Res., 2002;38:87
Characterization of the adrenoreceptor activities of isoprenaline in the field stimulated rat vas deferens: selective supersensitivity to beta 2-mediated responses following reserpine treatment.
Lotti et al.
β2-adrenergic receptor activates extracellular signal-regulated kinases (ERKs) via the small G protein Rap1 and the serine/threonine kinase B-Raf.
Schmitt and Stork
Characterization of the adrenoreceptor activities of isoprenaline in the field stimulated rat vas deferens: selective supersensitivity to beta 2-mediated responses following rese. treatment.
Lotti et al.
Citations for Isoproterenol hydrochloride
The citations listed below are publications that use Tocris products. Selected citations for Isoproterenol hydrochloride include:
27 Citations: Showing 1 - 10
Increased clusterin levels after myocardial infarction is due to a defect in protein degradation systems activity.
Authors: Turkieh Et al.
Cell Death Dis 2019;10:608
Female Mice Have Higher Angiogenesis in Perigonadal Adipose Tissue Than Males in Response to High-Fat Diet.
Authors: Rudnicki Et al.
Front Physiol 2018;9:1452
An Automated Platform for Assessment of Congenital and Drug-Induced Arrhythmia with hiPSC-Derived Cardiomyocytes.
Authors: McKeithan Et al.
Front Physiol 2017;8:766
Resveratrol enhances the inotropic effect but inhibits the proarrhythmic effect of sympathomimetic agents in rat myocardium.
Structural and Functional Analysis of a β2-Adrenergic Receptor Complex with GRK5.
Authors: Komolov Et al.
DMP-1-mediated Ghr gene recombination compromises skeletal development and impairs skeletal response to intermittent PTH.
Authors: Liu Et al.
FASEB J 2016;30:635
Arrhythmogenic remodeling of β2 versus β1 adrenergic signaling in the human failing heart.
Authors: Lang Et al.
Cereb Cortex 2015;8:409
Optodynamic simulation of β-adrenergic receptor signalling.
Authors: Siuda Et al.
Circ Arrhythm Electrophysiol 2015;6:8480
Adrenergic DNA damage of embryonic pluripotent cells via β2 receptor signalling.
Authors: Sun Et al.
Nat Commun 2015;5:15950
Locus Coeruleus Stimulation Facilitates Long-Term Depression in the Dentate Gyrus That Requires Activation of β-Adrenergic Receptors.
Authors: Hansen and Manahan-Vaughan
Eur J Neurosci 2015;25:1889
Application of BRET to monitor ligand binding to GPCRs.
Authors: Stoddart Et al.
Nat Methods 2015;12:661
Glucagon Increases Beating Rate but Not Contractility in Rat Right Atrium. Comparison with isoprote.
Authors: Merino Et al.
J Mol Cell Cardiol 2015;10:e0132884
Excitatory drive onto DArgic neurons in the rostral linear nucleus is enhanced by NE in an α1 adrenergic receptor-dependent manner.
Authors: Williams Et al.
PKA catalytic subunit compartmentation regulates contractile and hypertrophic responses to β-adrenergic signaling.
Authors: Yang Et al.
Inhibitory interactions between phosphorylation sites in the C terminus of α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor GluA1 subunits.
Authors: Gray Et al.
Biochem Biophys Res Commun 2014;289:14600
NE drives persistent activity in prefrontal cortex via synergistic α1 and α2 adrenoceptors.
Authors: Zhang Et al.
PLoS One 2013;8:e66122
Corticotropin releasing factor and catecholamines enhance glutamatergic neurotransmission in the lateral subdivision of the central amygdala.
Authors: Silberman and Winder
Resonant waveguide grating biosensor-enabled label-free and fluorescence detection of cell adhesion.
Authors: Zaytseva Et al.
Sens Actuators B Chem 2013;188
Age-related impairment in Ins release: the essential role of β(2)-adrenergic receptor.
Authors: Santulli Et al.
J Cell Biol 2012;61:692
Modulation of calcium-dependent inactivation of L-type Ca2+ channels via β-adrenergic signaling in thalamocortical relay neurons.
Authors: Rankovic Et al.
PLoS One 2011;6:e27474
β-Adrenergic receptors enhance excitatory transmission in the bed nucleus of the stria terminalis through a corticotrophin-releasing factor receptor-dependent and cocaine-regulated mechanism.
Authors: Nobis Et al.
Biol Psychiatry 2011;69:1083
Real-time analysis of the inside-out regulation of lymphocyte function-associated antigen-1 revealed similarities to and differences from very late antigen-4.
Authors: Chigaev Et al.
J Biol Chem 2011;286:20375
NE causes a biphasic change in mammalian pinealocye membrane potential: role of alpha1B-adrenoreceptors, phospholipase C, and Ca2+.
Authors: Zemkova Et al.
Reversibly sealed multilayer microfluidic device for integrated cell perfusion and on-line chemical analysis of cultured adipocyte secretions.
Authors: Clark Et al.
Anal Bioanal Chem 2010;397:2939
The effects of aging and chronic fluox. treatment on circadian rhythms and suprachiasmatic nucleus expression of neuropeptide genes and 5-HT1B receptors.
Authors: Duncan Et al.
J Biol Chem 2010;31:1646
Bitter peptides activate hTAS2Rs, the human bitter receptors.
Authors: Maehashi Et al.
PLoS One 2008;365:851
Noradrenergic inputs mediate state dependence of auditory responses in the avian song system.
Authors: Cardin and Schmidt
Sci Rep 2004;24:7745
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HEK293 cells transfected with cAMP FRET sensor, Epac-S(H187) were perfused in normal tyrode solution and excited alternately at 430 nm and fluorescence was captured at 470 and 535 nm. Isoprenaline (10 µM) was added at 1 min to elevate intracellular cAMP, which is detected as an increase in 470/535 fluorescence ratio.
Live assay treatment
We used this product to treat ARKO mice to investigate the cell signalling pathway