Chemical Name: 4-[1-Hydroxy-2-[(1-methylethyl)amino]ethyl]-1,2-benzenediol hydrochloride
Biological ActivityStandard selective β-adrenoceptor agonist; vasorelaxant and bronchodilator. Activation of β2 receptors activates downstream PKA and ERK, and may stimulate NO-mediated endothelium-dependent smooth muscle relaxation. Active in vivo. Also available as part of the β-Adrenoceptor Agonist Tocriset™ and Mixed Adrenergic Tocriset™.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Nitric oxide (NO) primarily accounts for endothelium-dependent component of β-adrenoceptor-activated smooth muscle relaxation of mouse aorta in response to isoprenaline.
Akimoto et al.
J.Smooth Muscle Res., 2002;38:87
Characterization of the adrenoreceptor activities of isoprenaline in the field stimulated rat vas deferens: selective supersensitivity to beta 2-mediated responses following reserpine treatment.
Lotti et al.
β2-adrenergic receptor activates extracellular signal-regulated kinases (ERKs) via the small G protein Rap1 and the serine/threonine kinase B-Raf.
Schmitt and Stork
Characterization of the adrenoreceptor activities of isoprenaline in the field stimulated rat vas deferens: selective supersensitivity to beta 2-mediated responses following rese. treatment.
Lotti et al.
Citations for Isoproterenol hydrochloride
The citations listed below are publications that use Tocris products. Selected citations for Isoproterenol hydrochloride include:
27 Citations: Showing 1 - 10
Increased clusterin levels after myocardial infarction is due to a defect in protein degradation systems activity.
Authors: Turkieh Et al.
Cell Death Dis 2019;10:608
Female Mice Have Higher Angiogenesis in Perigonadal Adipose Tissue Than Males in Response to High-Fat Diet.
Authors: Rudnicki Et al.
Front Physiol 2018;9:1452
An Automated Platform for Assessment of Congenital and Drug-Induced Arrhythmia with hiPSC-Derived Cardiomyocytes.
Authors: McKeithan Et al.
Front Physiol 2017;8:766
Resveratrol enhances the inotropic effect but inhibits the proarrhythmic effect of sympathomimetic agents in rat myocardium.
Structural and Functional Analysis of a β2-Adrenergic Receptor Complex with GRK5.
Authors: Komolov Et al.
DMP-1-mediated Ghr gene recombination compromises skeletal development and impairs skeletal response to intermittent PTH.
Authors: Liu Et al.
FASEB J 2016;30:635
Arrhythmogenic remodeling of β2 versus β1 adrenergic signaling in the human failing heart.
Authors: Lang Et al.
Cereb Cortex 2015;8:409
Optodynamic simulation of β-adrenergic receptor signalling.
Authors: Siuda Et al.
Circ Arrhythm Electrophysiol 2015;6:8480
Adrenergic DNA damage of embryonic pluripotent cells via β2 receptor signalling.
Authors: Sun Et al.
Nat Commun 2015;5:15950
Locus Coeruleus Stimulation Facilitates Long-Term Depression in the Dentate Gyrus That Requires Activation of β-Adrenergic Receptors.
Authors: Hansen and Manahan-Vaughan
Eur J Neurosci 2015;25:1889
Application of BRET to monitor ligand binding to GPCRs.
Authors: Stoddart Et al.
Nat Methods 2015;12:661
Glucagon Increases Beating Rate but Not Contractility in Rat Right Atrium. Comparison with isoprote.
Authors: Merino Et al.
J Mol Cell Cardiol 2015;10:e0132884
Excitatory drive onto DArgic neurons in the rostral linear nucleus is enhanced by NE in an α1 adrenergic receptor-dependent manner.
Authors: Williams Et al.
PKA catalytic subunit compartmentation regulates contractile and hypertrophic responses to β-adrenergic signaling.
Authors: Yang Et al.
Inhibitory interactions between phosphorylation sites in the C terminus of α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor GluA1 subunits.
Authors: Gray Et al.
Biochem Biophys Res Commun 2014;289:14600
NE drives persistent activity in prefrontal cortex via synergistic α1 and α2 adrenoceptors.
Authors: Zhang Et al.
PLoS One 2013;8:e66122
Corticotropin releasing factor and catecholamines enhance glutamatergic neurotransmission in the lateral subdivision of the central amygdala.
Authors: Silberman and Winder
Resonant waveguide grating biosensor-enabled label-free and fluorescence detection of cell adhesion.
Authors: Zaytseva Et al.
Sens Actuators B Chem 2013;188
Age-related impairment in Ins release: the essential role of β(2)-adrenergic receptor.
Authors: Santulli Et al.
J Cell Biol 2012;61:692
Modulation of calcium-dependent inactivation of L-type Ca2+ channels via β-adrenergic signaling in thalamocortical relay neurons.
Authors: Rankovic Et al.
PLoS One 2011;6:e27474
β-Adrenergic receptors enhance excitatory transmission in the bed nucleus of the stria terminalis through a corticotrophin-releasing factor receptor-dependent and cocaine-regulated mechanism.
Authors: Nobis Et al.
Biol Psychiatry 2011;69:1083
Real-time analysis of the inside-out regulation of lymphocyte function-associated antigen-1 revealed similarities to and differences from very late antigen-4.
Authors: Chigaev Et al.
J Biol Chem 2011;286:20375
NE causes a biphasic change in mammalian pinealocye membrane potential: role of alpha1B-adrenoreceptors, phospholipase C, and Ca2+.
Authors: Zemkova Et al.
Reversibly sealed multilayer microfluidic device for integrated cell perfusion and on-line chemical analysis of cultured adipocyte secretions.
Authors: Clark Et al.
Anal Bioanal Chem 2010;397:2939
The effects of aging and chronic fluox. treatment on circadian rhythms and suprachiasmatic nucleus expression of neuropeptide genes and 5-HT1B receptors.
Authors: Duncan Et al.
J Biol Chem 2010;31:1646
Bitter peptides activate hTAS2Rs, the human bitter receptors.
Authors: Maehashi Et al.
PLoS One 2008;365:851
Noradrenergic inputs mediate state dependence of auditory responses in the avian song system.
Authors: Cardin and Schmidt
Sci Rep 2004;24:7745
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HEK293 cells transfected with cAMP FRET sensor, Epac-S(H187) were perfused in normal tyrode solution and excited alternately at 430 nm and fluorescence was captured at 470 and 535 nm. Isoprenaline (10 µM) was added at 1 min to elevate intracellular cAMP, which is detected as an increase in 470/535 fluorescence ratio.
Live assay treatment
We used this product to treat ARKO mice to investigate the cell signalling pathway