Isoproterenol hydrochloride

Tocris Bioscience | Catalog # 1747

Standard selective β agonist
Tocris Bioscience

Key Product Details

Description

Standard selective β agonist

Alternative Names

Isoprenaline
View all Non-selective Adrenergic beta Receptor Agonists »

Product Description

Isoproterenol hydrochloride is a standard selective β-adrenoceptor agonist; vasorelaxant and bronchodilator. Activation of β2 receptors activates downstream PKA and ERK, and may stimulate NO-mediated endothelium-dependent smooth muscle relaxation. Active in vivo.

Scientific Data Images for Isoproterenol hydrochloride

Isoproterenol affects the rate of cardiomyocyte contraction

Small molecules affect the rate of cardiomyocyte contraction.

Cardiomyocytes were differentiated from the BG01V human embryonic stem cells and assessed for their ability to contract using the Fluo-4 calcium binding assay. Rate interval graph show fluctuations in Fluo-4 fluorescence intensity, indicative of cardiomyocyte contraction, under basal conditions (brown; untreated) and following sequential treatment with Isoproterenol (blue; Catalog # 1747), a beta -adrenergic agonist, and Propranolol (tan; Catalog # 0624), a beta -adrenergic antagonist. Table shows drug concentrations and contraction rate of cardiomyocytes during each treatment. The contraction rate of differentiated cardiomyocytes was determined as the number of contractions (contraction-interval plus relaxation interval) per minute.

Product Specifications for Isoproterenol hydrochloride

Molecular Weight

247.72

Formula

C11H17NO3.HCl

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

4-[1-Hydroxy-2-[(1-methylethyl)amino]ethyl]-1,2-benzenediol hydrochloride

CAS Number

51-30-9

PubChem ID

5807

InChI Key

IROWCYIEJAOFOW-UHFFFAOYSA-N

SMILES

Cl.CC(C)NCC(O)C1=CC(O)=C(O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 24.77 100

Preparing Stock Solutions for Isoproterenol hydrochloride

The following data is based on the product molecular weight 247.72.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.04 mL 20.18 mL 40.37 mL
5 mM 0.81 mL 4.04 mL 8.07 mL
10 mM 0.40 mL 2.02 mL 4.04 mL
50 mM 0.08 mL 0.40 mL 0.81 mL

Calculators

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Product Documents for Isoproterenol hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Isoproterenol hydrochloride

For research use only

Citations for Isoproterenol hydrochloride

Customer Reviews for Isoproterenol hydrochloride (4)

4.8 out of 5
4 Customer Ratings
5 Stars
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4 Stars
25%
3 Stars
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2 Stars
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1 Stars
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  • Use it to induce Mg influx
    Name: Yuyang Sun
    Species: Human
    Cell Line/Tissue: sh-sy5y
    Verified Customer | Posted 06/29/2022
    Used it in Mg image experiment, and it can induce obvious Mg influx.
    Isoproterenol hydrochloride 1747
  • Isoprenaline elevates intracellular cAMP
    Name: Niroj Shrestha
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 07/08/2019
    HEK293 cells transfected with cAMP FRET sensor, Epac-S(H187) were perfused in normal tyrode solution and excited alternately at 430 nm and fluorescence was captured at 470 and 535 nm. Isoprenaline (10 µM) was added at 1 min to elevate intracellular cAMP, which is detected as an increase in 470/535 fluorescence ratio.
    Isoproterenol hydrochloride 1747
  • Beta agonist
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 09/20/2018
    Live assay treatment
  • Isoproterenol hydrochloride treated ARKO mice
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Verified Customer | Posted 01/05/2018
    We used this product to treat ARKO mice to investigate the cell signalling pathway
    Isoproterenol hydrochloride 1747

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