ITK Antibody

Novus Biologicals | Catalog # NB100-807

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ITK Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RLLRQLAEIAESGL corresponding to C-Terminus according to NP_005537.3.

Localization

Plasma membrane

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat ITK Antibody (NB100-807) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-ITK Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ITK Antibody

Western Blot: ITK Antibody [NB100-807]

Western Blot: ITK Antibody [NB100-807]

Western Blot: ITK Antibody [NB100-807] - Staining of Jurkat nuclear cell lysate with antibody at 1 ug/mL (A) and negative control Human parathyroid gland (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ITK Antibody [NB100-807]

Immunocytochemistry/ Immunofluorescence: ITK Antibody [NB100-807]

Immunocytochemistry/Immunofluorescence: ITK Antibody [NB100-807] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong cytoplasmic and weak nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: ITK Antibody [NB100-807]

Immunocytochemistry/ Immunofluorescence: ITK Antibody [NB100-807]

Immunocytochemistry/Immunofluorescence: ITK Antibody [NB100-807] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for ITK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 75 kDa band bands observed in Jurkat Lysates.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ITK

ITK is a T cell signaling protein. The Tec family of non receptor tyrosine kinases is composed of six proteins designated Tec, ITK (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. ITK associates with CD28 and becomes activated subsequent to CD28 ligation.

Long Name

IL-2-inducible T-cell Kinase

Alternate Names

EMT, LYK, PSCTK2, Tcsk

Entrez Gene IDs

3702 (Human); 16428 (Mouse)

Gene Symbol

ITK

UniProt

NP_005537 (Human)

Additional ITK Products

Product Documents for ITK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ITK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Natural Killer T (NKT) Cells

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Protocols

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