IVD Antibody (04) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-06536

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 04

Format

Azide and BSA Free
View all IVD Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IVD (Accession#: AAH17202.1; His30-His423).

Reactivity Notes

No cross-reactivity in ELISA with:

Insect cell lysate

Specificity

No cross-reactivity in ELISA with: Insect cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IVD Antibody (04) - Azide and BSA Free

Western Blot: IVD Antibody (04) [NBP3-06536]

Western Blot: IVD Antibody (04) [NBP3-06536]

Western Blot: IVD Antibody (04) [NBP3-06536] - Lane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:40 kDa
Flow Cytometry: IVD Antibody (04) [NBP3-06536]

Flow Cytometry: IVD Antibody (04) [NBP3-06536]

Flow Cytometry: IVD Antibody (04) [NBP3-06536] - Analysis of Human IVD expression on HeLa cells. The cells were treated, stained with purified anti-Human IVD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunoprecipitation: IVD Antibody (04) [NBP3-06536]

Immunoprecipitation: IVD Antibody (04) [NBP3-06536]

Immunoprecipitation: IVD Antibody (04) [NBP3-06536] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-IVD mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IVD mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa
IVD Antibody (04) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IVD Antibody (04) [NBP3-06536] -

Immunocytochemistry/Immunofluorescence: IVD Antibody (04) [NBP3-06536] - Staining of Human IVD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-06536 antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for IVD Antibody (04) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

0.1-0.5 uL/mg of lysate

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IVD

Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ACAD2, EC 1.3.99, EC 1.3.99.10, FLJ12715, FLJ34849, isovaleryl-CoA dehydrogenase, mitochondrial

Gene Symbol

IVD

Additional IVD Products

Product Documents for IVD Antibody (04) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IVD Antibody (04) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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