IZUMO1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83086

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Porcine

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IZUMO1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQM

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IZUMO1 Antibody - BSA Free

Western Blot: IZUMO1 Antibody [NBP1-83086]

Western Blot: IZUMO1 Antibody [NBP1-83086]

Western Blot: IZUMO1 Antibody [NBP1-83086] - Protein extracts from four different pig ejaculates and a human sample. Lanes F: fresh sperm. Lanes F-T: frozen-thawed sperm. Lanes H: human fresh sperm. Image submitted by a verified customer review.
Western Blot: IZUMO1 Antibody [NBP1-83086]

Western Blot: IZUMO1 Antibody [NBP1-83086]

Western Blot: IZUMO1 Antibody [NBP1-83086] - Analysis in control (vector only transfected HEK293T lysate) and IZUMO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Analysis in human testis and liver tissues. Corresponding IZUMO1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Staining of human duodenum shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086]

Immunohistochemistry-Paraffin: IZUMO1 Antibody [NBP1-83086] - Staining of human testis shows weak to moderate cytoplasmic positivity in spermatids.

Applications for IZUMO1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunocytochemistry reported in scientific literature (PMID:31391940). IZUMO1 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-83086 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IZUMO1

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasmamembrane binding and fusion (Inoue et al., 2005 (PubMed 15759005)).(supplied by OMIM)

Long Name

Izumo Sperm-egg Fusion 1

Alternate Names

OBF

Gene Symbol

IZUMO1

Additional IZUMO1 Products

Product Documents for IZUMO1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IZUMO1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IZUMO1 Antibody - BSA Free

Customer Reviews for IZUMO1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • IZUMO1 Antibody
    Name: Marc Llavanera
    Application: Western Blot
    Sample Tested: Sperm
    Species: Pig and Human
    Verified Customer | Posted 07/10/2019
    Protein extracts from four different pig ejaculates and a human sample were loaded onto the gel. Lanes F: fresh sperm. Lanes F-T: frozen-thawed sperm. Lanes H: human fresh sperm.
    IZUMO1 Antibody - BSA Free NBP1-83086

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Protocols

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