Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Insect

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Microarray

Cited:

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 53B7

Format

BSA Free
View all Jak2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human JAK2 (1014-1132aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Jak2 Antibody (53B7) - BSA Free

Western Blot: Jak2 Antibody (53B7)BSA Free [NBP2-59451]

Western Blot: Jak2 Antibody (53B7)BSA Free [NBP2-59451]

Western Blot: Jak2 Antibody (53B7) [NBP2-59451] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of JAK2 was detected. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Immunocytochemistry/ Immunofluorescence: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Immunocytochemistry/Immunofluorescence: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) and anti-human JAK2 antibody at 1:100 with goat anti-mouse IgG Alexa Fluor 488 conjugate (Green).
Flow Cytometry: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Flow Cytometry: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Flow Cytometry: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cell line, antibody at 2 - 5 ug per 10^6cells (red line). Secondary antibody: goat anti-mouse IgG Alexa Fluor 488 conjugate. Isotype control antibody: mouse IgG (black line).
Western Blot: Jak2 Antibody (53B7)BSA Free [NBP2-59451]

Western Blot: Jak2 Antibody (53B7)BSA Free [NBP2-59451]

Western Blot: Jak2 Antibody (53B7) [NBP2-59451] - Lane 1: K-562 cell lysates, Lane 2: TF-1 cell lysates, Lane 3: 293T cell lysates, Lane 4: Jurkat cell lysates, Lane 5: THP-1 cell lysates
Microarray: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Microarray: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Microarray: Jak2 Antibody (53B7) [NBP2-59451] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verifed customer review.

Applications for Jak2 Antibody (53B7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Simple Western

1:20

Western Blot

1:1000
Application Notes

This Jak2 Antibody (53B7) is validated for microarray from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in KB, separated by Size, antibody dilution of 1:20

Reviewed Applications

Read 3 reviews rated 5 using NBP2-59451 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Jak2

Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Gene Symbol

JAK2

Additional Jak2 Products

Product Documents for Jak2 Antibody (53B7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Jak2 Antibody (53B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Jak2 Antibody (53B7) - BSA Free

Customer Reviews for Jak2 Antibody (53B7) - BSA Free (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
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3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Jak2 Antibody (53B7)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: TF-1 human erythroleukemic cell line
    Species: Human
    Verified Customer | Posted 12/06/2021
    Jak2 Antibody in TF-1 human erythroleukemic cell line
    Jak2 Antibody (53B7) - BSA Free NBP2-59451
  • Jak2 Antibody (53B7)
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Jak2 Antibody (53B7) - BSA Free NBP2-59451
  • Jak2 Antibody (53B7)
    Name: Jie Lan
    Application: Western Blot
    Sample Tested: MCF7 cell line
    Species: Human
    Verified Customer | Posted 12/02/2018
    MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of JAK2 was detected through western blot.
    Jak2 Antibody (53B7) - BSA Free NBP2-59451

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Protocols

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