JSRP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81998

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all JSRP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EAEVRPKIPGSREAAENDEEEPGEATGEAVREDRVTLADRGPKERPRREGKPRKEKPRKEERPKKERPRKEER

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JSRP1 Antibody - BSA Free

Western Blot: JSRP1 Antibody [NBP1-81998]

Western Blot: JSRP1 Antibody [NBP1-81998]

Western Blot: JSRP1 Antibody [NBP1-81998] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Skeletal muscle tissue
Immunocytochemistry/ Immunofluorescence: JSRP1 Antibody [NBP1-81998]

Immunocytochemistry/ Immunofluorescence: JSRP1 Antibody [NBP1-81998]

Immunocytochemistry/Immunofluorescence: JSRP1 Antibody [NBP1-81998] - Staining of human cell line RH-30 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998]

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998]

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998] - Staining in human skeletal muscle and heart muscle tissues using anti-JSRP1 antibody. Corresponding JSRP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998]

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998]

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998] - Staining of human heart muscle shows low expression as expected.
Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998]

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998]

Immunohistochemistry-Paraffin: JSRP1 Antibody [NBP1-81998] - Staining of human skeletal muscle shows high expression.

Applications for JSRP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JSRP1

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR®s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR®s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR®s and RyR®s. Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.

Alternate Names

FLJ32416, JP45, JP-45homolog of mouse skeletal muscle sarcoplasmic reticulum protein JP-45, junctional sarcoplasmic reticulum protein 1,2310032K21Rik, Junctional-face membrane protein of 45 kDa homolog, skeletal muscle sarcoplasmic reticulum protein JP-45

Gene Symbol

JSRP1

Additional JSRP1 Products

Product Documents for JSRP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for JSRP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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