JunD Antibody (2D5A1)

Name: JunD Antibody (2D5A1)
Brand: Novus Biologicals
SKU: NBP3-16602
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16602

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2D5A1 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human JunD (P17535). METPFYGDEALSGLGGGASGSGGSFASPGRLFPGAPPTAAAGSMMKKDALTLSLSEQVAAALKPAAAPPPTPLRADGAPSAAPPDGLLASPDLGLLKLASPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JunD Antibody (2D5A1)

Western Blot: JunD Antibody (2D5A1) [NBP3-16602]

Western Blot: JunD Antibody (2D5A1) [NBP3-16602] - Western blot analysis of extracts of various cell lines, using JunD antibody (NBP3-16602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation: JunD Antibody (2D5A1) [NBP3-16602]

Immunoprecipitation: JunD Antibody (2D5A1) [NBP3-16602] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug JunD antibody (NBP3-16602). Western blot was performed from the immunoprecipitate using JunD antibody (NBP3-16602) at a dilition of 1:1000.

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] -

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] - Immunohistochemistry analysis of JunD in paraffin-embedded human thyroid tissue using JunD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] -

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] - Immunohistochemistry analysis of JunD in paraffin-embedded human small intestine tissue using JunD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] -

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] - Immunohistochemistry analysis of JunD in paraffin-embedded human esophagus tissue using JunD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] -

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] - Immunohistochemistry analysis of JunD in paraffin-embedded rat colon tissue using JunD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] -

Immunohistochemistry: JunD Antibody (2D5A1) [NBP3-16602] - Immunohistochemistry analysis of JunD in paraffin-embedded mouse colon tissue using JunD Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

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Applications for JunD Antibody (2D5A1)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JunD

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Gene Symbol

JUND

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Product Documents for JunD Antibody (2D5A1)

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Certificate of Analysis

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Product Specific Notices for JunD Antibody (2D5A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.