KAT3B/p300 Antibody (RW105) [DyLight 405]

Novus Biologicals | Catalog # NB100-616V

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Mustelid, Primate

Applications

Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), In vitro assay, Electron Microscopy, Knockdown Validated

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RW105
View all p300 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Reactivity Notes

Human, mouse, rat, monkey, and mink.

Localization

Nuclear

Specificity

This is specific for p300 protein. This recognizes residues 1921-2023.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for KAT3B/p300 Antibody (RW105) [DyLight 405]

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

In vitro assay

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This KAT3B/p300 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. In Western blot a band is seen at ~300 kDa, representing the p300 protein. Lower non-specific MW bands (~45-66 kDa) ay be seen with longer exposure times. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: p300

In eukaryotic genome, the chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and it is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Long Name

E1A-binding Protein p300

Alternate Names

EP300

Gene Symbol

EP300

Additional p300 Products

Product Documents for KAT3B/p300 Antibody (RW105) [DyLight 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KAT3B/p300 Antibody (RW105) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Notch Pathway
p53 Pathway

Customer Reviews for KAT3B/p300 Antibody (RW105) [DyLight 405]

There are currently no reviews for this product. Be the first to review KAT3B/p300 Antibody (RW105) [DyLight 405] and earn rewards!

Have you used KAT3B/p300 Antibody (RW105) [DyLight 405]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies