Keap1 Antibody (OTI1B4)

Novus Biologicals | Catalog # NBP2-03319

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B4
View all Keap1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

69.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Keap1 Antibody (OTI1B4)

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
Immunocytochemistry/ Immunofluorescence: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunocytochemistry/ Immunofluorescence: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunocytochemistry/Immunofluorescence: Keap1 Antibody (OTI1B4) [NBP2-03319] - Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY KEAP1 using anti-KEAP1 antibody (green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319]

Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319]

Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed by flow cytometry.
Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues used at a 1:200 dilution.
Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of human BEAS2B cells.
Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319]

Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and KEAP1-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-beta-actin antibody as a loading control (1:500).
Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319]

Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody.
Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319]

Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319]

Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Hela cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319]

Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319]

Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Jurkat cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Keap1 Antibody (OTI1B4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Keap1

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq].

Long Name

Kelch-like ECH-associated Protein 1

Alternate Names

INrf2

Entrez Gene IDs

9817 (Human)

Gene Symbol

KEAP1

Additional Keap1 Products

Product Documents for Keap1 Antibody (OTI1B4)

Certificate of Analysis

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Product Specific Notices for Keap1 Antibody (OTI1B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Keap1 Antibody (OTI1B4)

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Protocols

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