KHSRP Antibody

Novus Biologicals | Catalog # NBP1-18910

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all KHSRP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human KH-type splicing regulatory protein using the numbering given in entry Q92945.3 (GeneID 8570).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25907681).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KHSRP Antibody

Simple Western: KHSRP Antibody [NBP1-18910]

Simple Western: KHSRP Antibody [NBP1-18910]

Simple Western: KHSRP Antibody [NBP1-18910] - Simple Western lane view shows a specific band for KHSRP in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: KHSRP Antibody [NBP1-18910]

Immunohistochemistry: KHSRP Antibody [NBP1-18910]

KHSRP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18910-img0009.jpg
Western Blot: KHSRP Antibody [NBP1-18910]

Western Blot: KHSRP Antibody [NBP1-18910]

Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. KSRP was also immunoprecipitated by rabbit anti-KSRP antibody NBP1-18911.
Immunohistochemistry-Frozen: KHSRP Antibody [NBP1-18910]

Immunohistochemistry-Frozen: KHSRP Antibody [NBP1-18910]

Immunohistochemistry-Frozen: KHSRP Antibody [NBP1-18910] - KSRP immunostaining in mouse cerebral cortex and hippocampus. Cell were stained with Novus KSRP. This KSRP antibody was also used for immunoprecipation and western blot analyses. Image from confirmed customer review.
Western Blot: KHSRP Antibody [NBP1-18910]

Western Blot: KHSRP Antibody [NBP1-18910]

Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiKSRP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910]

Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910]

Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-KSRP used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910]

Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910]

Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-KSRP used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
KHSRP Antibody

Immunohistochemistry: KHSRP Antibody [NBP1-18910] -

Immunofluorescence of ILF3 and KHSRP in the retina. Retinal sections were prepared from control mice and from mice at one day after light exposure and stained (A) for glutamine synthetase (GS, green) and ILF3 (red) or (B) for GS (green) and KHSRP (red). (C) Control stainings with secondary antibodies only (as indicated). DAPI was used to visualize nuclei. DAPI, 4',6-diamidino-2-phenylindole.
KHSRP Antibody

Western Blot: KHSRP Antibody [NBP1-18910] -

Effect of MnCl2 and LPS on protein levels of KHSRP and NLRP3 quantitated by Western blot analysis 24 h post-exposure, relative band intensity quantified (a) Western blot analysis for the impact of MnCl2 exposure on NLRP3, KHSRP, GAPDH, (b) fold change in KHSRP protein levels (relative quantification) post-MnCl2 exposure, (c) fold change in NLRP3 protein post-MnCl2 exposure, (d) Western blot analysis for the impact of LPS exposure on NLRP3, KHSRP, GAPDH, (e) fold change in KHSRP protein levels (relative quantification) post-LPS exposure, (f) fold change in NLRP3 protein post-LPS exposure. Protein intensity values were normalized to the housekeeping protein, GAPDH, and expressed as a fold-induction over the control sample (set at a value of 1). Data represented as mean +/- SEM. * p < 0.05, ** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36362011), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KHSRP Antibody

Immunocytochemistry/ Immunofluorescence: KHSRP Antibody [NBP1-18910] -

Immunofluorescence of ILF3 and KHSRP in the retina. Retinal sections were prepared from control mice and from mice at one day after light exposure and stained (A) for glutamine synthetase (GS, green) and ILF3 (red) or (B) for GS (green) and KHSRP (red). (C) Control stainings with secondary antibodies only (as indicated). DAPI was used to visualize nuclei. DAPI, 4',6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25907681), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for KHSRP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reactivity reported in (PMID: 25907681)

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Frozen

Reactivity reported form a verified customer review

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

2-10 ug/mg lysate

Simple Western

1:40

Western Blot

1:2000-1:10000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:40, apparent MW was 114 kDa.

Reviewed Applications

Read 1 review rated 5 using NBP1-18910 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KHSRP

KH-type splicing regulatory protein (KSRP) contains four K homology RNA-binding domains. KH domains are found in a wide variety of proteins including ribosomal proteins, transcription factors, and mRNA processing proteins. KSRP has been found to promote the degradation of mRNAs that encode proteins involved in proliferation and the inflammatory response. Alternative names for KSRP include Far upstream element-binding protein 2, FUSE-binding protein 2, p75 and FUBP2.

Alternate Names

FBP2far upstream element-binding protein 2, FUBP2p75, FUSE binding protein 2, FUSE-binding protein 2, KH type-splicing regulatory protein, KH-type splicing regulatory protein, KSRPMGC99676

Entrez Gene IDs

8570 (Human); 16549 (Mouse)

Gene Symbol

KHSRP

UniProt

Q92945.3 (Human)

Additional KHSRP Products

Product Documents for KHSRP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KHSRP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for KHSRP Antibody (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: mouse brain
    Species: Mouse
    Verified Customer | Posted 02/24/2014
    KSRP immunostaining in mouse tissues
    KHSRP Antibody NBP1-18910

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