Kir2.1 Antibody (JE54-56)

Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505]

Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution.

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 30s;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Kir2.1 Antibody (JE54-56) [NBP3-32505]

Immunohistochemistry: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Kir2.1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32505, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (JE54-56) [NBP3-32505]

Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (JE54-56) [NBP3-32505] - ICC staining of Kir2.1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32505, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).