KLF4 Antibody (CL5782) - BSA Free

Novus Biologicals | Catalog # NBP2-61426

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL5782

Format

BSA Free
View all KLF4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from O43474, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Mouse, Rat (89%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for KLF4 Antibody (CL5782) - BSA Free

Western Blot: KLF4 Antibody (CL5782) [NBP2-61426]

Western Blot: KLF4 Antibody (CL5782) [NBP2-61426]

Western Blot: KLF4 Antibody (CL5782) [NBP2-61426] - Analysis in human cell line U-2197.
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426]

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426]

Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of U-2197cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426]

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426]

Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of PC-3 cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human rectum shows strong nuclear immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human placenta shows nuclear positivity in the trophoblast.
Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human prostate shows nuclear immunoreactivity in glandular cells.

Applications for KLF4 Antibody (CL5782) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Gene Symbol

KLF4

Additional KLF4 Products

Product Documents for KLF4 Antibody (CL5782) - BSA Free

Certificate of Analysis

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Product Specific Notices for KLF4 Antibody (CL5782) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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