Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-19320

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (91%), Chimpanzee (100%), Porcine (90%), Rabbit (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Lactate Dehydrogenase A/LDHA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lactate Dehydrogenase A/LDHA. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (80%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Human lung papillory adenocarcinoma, using LDHA antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] -

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (NBP2-19320) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] -

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (NBP2-19320) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] -

LDHA antibody detects LDHA protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] -

LDHA antibody detects LDHA protein by western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (NBP2-19320) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] -

LDHA antibody detects LDHA protein by western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
C. 30 ug NIH-3T3 whole cell lysate/extract
D. 30 ug Raw264.7 whole cell lysate/extract
E. 30 ug C2C12 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (NBP2-19320) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase A/LDHA

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

UniProt

P00338 (Human)

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lactate Dehydrogenase A/LDHA Antibody - Azide Free

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Protocols

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