Lamin A + C Antibody (4L8Q0)

Novus Biologicals | Catalog # NBP3-15754

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4L8Q0 expressed in HEK293
View all Lamin A + C Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A + C (NP_733821.1). SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin A + C Antibody (4L8Q0)

Knockout Validated: Lamin A + C Antibody (4L8Q0) [NBP3-15754]

Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754]

Western Blot: Lamin A + C Antibody (4L8Q0) [NBP3-15754]- Western blot analysis of extracts from normal (control) and Lamin A + C knockout (KO) HeLa cells, using Lamin A + C antibody (NBP3-15754) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754]

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754]

Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunofluorescence analysis of U2OS cells using Lamin A + C antibody (NBP3-15754) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.
Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754]

Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754]

Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [NBP3-15754] - Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A + C antibody (NBP3-15754). Western blot was performed from the immunoprecipitate using Lamin A + C antibody (NBP3-15754) at a dilition of 1:20000.
Lamin A + C Antibody (4L8Q0)

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Lamin A + C Antibody (4L8Q0)

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Lamin A + C Antibody (4L8Q0)

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Lamin A + C Antibody (4L8Q0)

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Confocal imaging of U-2 OS cells using [KO Validated] Lamin A + C Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Lamin A + C Antibody (4L8Q0)

Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunoprecipitation: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunoprecipitation analysis of 300 ug extracts from PC-12 cells using 3 ug [KO Validated] Lamin A + C Rabbit mAb. Western blot was performed from the immunoprecipitate using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:100000.
Lamin A + C Antibody (4L8Q0)

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Lamin A + C Antibody (4L8Q0)

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - The STORM super-resolution (SR) imaging of U-2 OS cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200 with 3% paraformaldehyde (PFA) +0.1% glutaraldehyde (GA) fixation. The immunostaining was performed by Full Automatic Immunofluorescence Workflow System (Workflow Ultra300, Nano-Micro imaging, China). Image was performed with Single-Molecule Localization Super-Resolution Microscopy (STORM Ultra300, Nano-Micro imaging, China). We acknowledge Nano-Micro imaging Biotechnology Co., Ltd. in SR image processing and kindly providing this image.
Lamin A + C Antibody (4L8Q0)

Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Western blot analysis of lysates from A549 cells using [KO Validated] Lamin A + C Rabbit mAb at 1:240000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Lamin A + C Antibody (4L8Q0)

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunofluorescence analysis of L929 cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200. Blue: DAPI for nuclear staining.
Lamin A + C Antibody (4L8Q0)

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunofluorescence analysis of H9C2 cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:200. Blue: DAPI for nuclear staining.
Lamin A + C Antibody (4L8Q0)

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Lamin A + C Antibody (4L8Q0)

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Confocal immunofluorescence analysis of HeLa cells using [KO Validated] Lamin A + C Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Lamin A + C Antibody (4L8Q0)

Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Western Blot: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Western blot analysis of various lysates using [KO Validated] Lamin A + C Rabbit mAb at 1:300000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Lamin A + C Antibody (4L8Q0)

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] -

Immunohistochemistry: Lamin A + C Antibody (4L8Q0) [Lamin A + C] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KO Validated] Lamin A + C Rabbit mAb at a dilution of 1:1300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Applications for Lamin A + C Antibody (4L8Q0)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:800

Immunohistochemistry

1:1000 - 1:4000

Immunohistochemistry-Paraffin

1:1000 - 1:4000

Immunoprecipitation

0.5ug-4ug antibody for 200ug-400ug extracts of whole cells

Western Blot

1:50000 - 1:300000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lamin A + C

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Alternate Names

CDCD1, CDDC, CMD1A, CMT2B1, dilated 1A (autosomal dominant), EMD2, FPLD, HGPSFPL, lamin A/C, lamin A/C-like 1, lamin-A/C, LDP1, LFP, LGMD1B, limb girdle muscular dystrophy 1B (autosomal dominant), LMN1IDC, LMNC, LMNL1, prelamin-A/C, PRO1,70 kDa lamin, progeria 1 (Hutchinson-Gilford type), renal carcinoma antigen NY-REN-32

Gene Symbol

LMNA

Additional Lamin A + C Products

Product Documents for Lamin A + C Antibody (4L8Q0)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lamin A + C Antibody (4L8Q0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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