Laminin Antibody (LAM-89)

Novus Biologicals | Catalog # NB600-883

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Porcine, Canine (Negative), Chicken (Negative), Feline, Fish, Goat (Negative), Guinea Pig (Negative), Rabbit (Negative), Rat (Negative), Reptile (Negative), Sheep (Negative)

Cited:

Human, Mouse, Fish

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LAM-89
View all Laminin Primary Antibodies »

Product Specifications

Immunogen

Laminin Antibody (LAM-89) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Reactivity Notes

Should not react with carp, lizard, frog, snake. Stains basal membranes of human, pig and cat. Fish reactivity reported in scientific literature (PMID: 26517388). Human and mouse reactivity reported in scientific literature.

Localization

Extracellular. Found in the basement membranes.

Marker

Basement Membrane Marker

Specificity

Laminin Antibody (LAM-89) specificity was determined by immunoperoxidase and immunofluorescent labeling of protease-digested, formalin-fixed, paraffin-embedded human or animal tissue sections, localizing specifically the basal membrane of blood vessels, epithelium, nerve, and muscle tissues. Localizes specifically in the basal membrane of blood vessels, epithelium, nerve and muscle fibers. Shows no cross-reaction with collagen type IV, fibronectin, vitronectin or chondroitin sulfates type A, B and C using a dot blot immunoassay. This antibody may be used for identification of laminin in various human tissues and bodily fluids.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

337 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin Antibody (LAM-89)

Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883]

Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883]

Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883] - Formalin-fixed, paraffin-embedded human tongue section, enzyme-treated was stained with 1:2,000 Monoclonal Anti-Laminin Clone: LAM-89. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883]

Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883]

Immunohistochemistry: Laminin Antibody (LAM-89) [NB600-883] - Analysis in human placenta section with Laminin Antibody (LAM-89) [NB600-883]

Applications for Laminin Antibody (LAM-89)

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000
Application Notes
Use in ICC/IF reported in scientific literature. Use in IHC-P reported in scientific literature (PMID: 26517388). Can be used for protease-digested formalin-fixed, paraffin embedded human or animal tissue sections. Localizes the basal membrane of blood vessels, epithelium, nerve, and muscle fibers. IHC-P: This concentration was determined by immunoperoxidase staining of protease-digested human tissue. Antigen unmasking is critical in using this product. Optimal dilutions/concentrations should be determined by the end user. indirect ELISA: suitable

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Alternate Names

LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha

Entrez Gene IDs

316758 (Rat)

Gene Symbol

LAMA1

Additional Laminin Products

Product Documents for Laminin Antibody (LAM-89)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Laminin Antibody (LAM-89)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Laminin Antibody (LAM-89)

Customer Reviews for Laminin Antibody (LAM-89)

There are currently no reviews for this product. Be the first to review Laminin Antibody (LAM-89) and earn rewards!

Have you used Laminin Antibody (LAM-89)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Laminin Antibody (LAM-89)

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for Antibodies