Laminin beta 1 Antibody (2D9G5)

Novus Biologicals | Catalog # NBP1-51558

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2D9G5
View all Laminin beta 1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Laminin beta 1 (aa31-270) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Laminin beta 1 Antibody (2D9G5)

Western Blot: Laminin beta 1 Antibody (2D9G5)BSA Free [NBP1-51558]

Western Blot: Laminin beta 1 Antibody (2D9G5)BSA Free [NBP1-51558]

Western Blot: Laminin beta 1 Antibody (2D9G5) [NBP1-51558] - Analysis using LAMB1 mouse mAb against truncated LAMB1-His recombinant protein (1).
Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (2D9G5) - BSA Free [NBP1-51558]

Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (2D9G5) - BSA Free [NBP1-51558]

Immunocytochemistry/Immunofluorescence: Laminin beta 1 Antibody (2D9G5) [NBP1-51558] - Human salivary cells express laminin beta 1 (red) and direct its secretion to the cell membrane in 3D hyaluronan gels. Image from verified customer review.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (2D9G5) - BSA Free [NBP1-51558]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (2D9G5) - BSA Free [NBP1-51558]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (2D9G5) [NBP1-51558] - Analysis of paraffin-embedded human Thyroid tissues using LAMB1 mouse mAb

Applications for Laminin beta 1 Antibody (2D9G5)

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
ICC/IF reported in verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-51558 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin beta 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing type IV collagen networks, nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

CLM, cutis laxa with marfanoid phenotype, Laminin B1 chain, laminin subunit beta-1, laminin, beta 1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, MGC142015

Gene Symbol

LAMB1

Additional Laminin beta 1 Products

Product Documents for Laminin beta 1 Antibody (2D9G5)

Certificate of Analysis

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Product Specific Notices for Laminin beta 1 Antibody (2D9G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Laminin beta 1 Antibody (2D9G5) (1)

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  • Laminin beta 1 Antibody (2D9G5)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Primary human salivary cells
    Species: Human
    Verified Customer | Posted 12/10/2018
    Human salivary cells express laminin beta 1 (red) and direct its secretion to the cell membrane in 3D hyaluronan gels.
    Used at a dilution of 1:100 Blocked with goat serum
    Laminin beta 1 Antibody (2D9G5) NBP1-51558

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Protocols

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