Laminin beta 3 Antibody (CL3353) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43799
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL3353
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q13751, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Laminin beta 3 Antibody (CL3353) - Azide and BSA Free
![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-2822025128379.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-282202512303870.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of human fallopian tube shows moderate to strong positivity in basement membrane of glandular cells.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-282202513121832.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of human pancreas shows no positivity in glandular cells as expected.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-28220251344266.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of human skin shows moderate positivity in the basement membrane of the basal layer of epidermal cells.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-28220251391988.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of human prostate shows moderate to strong positivity in basement membrane of glandular cells.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-2822025133580.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of rat lung shows moderate to strong positivity in basement membrane of pneumocytes.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Western Blot: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-28220251337862.jpg "Western Blot: Laminin beta 3 Antibody [NBP3-43799]")
Western Blot: Laminin beta 3 Antibody [NBP3-43799]
Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-282202513254789.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of rat skin shows moderate to strong positivity in basement membrane basal epidermal cells.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-28220251337843.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of rat kidney shows strong positivity in basement membrane in cells in glomeruli.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-282202513381544.jpg "Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]")
Immunohistochemistry-Paraffin: Laminin beta 3 Antibody [NBP3-43799]
Staining of rat rectum shows moderate positivity in basement membrane of glandular cells, as well as strong positivity in endothelial cells.![Laminin beta 3 Antibody (CL3353) - Azide and BSA Free Western Blot: Laminin beta 3 Antibody [NBP3-43799]](https://resources.rndsystems.com/images/products/nbp3-43799_mouse-laminin-beta-3-mab-cl3353-azide-and-bsa-free-282202513355834.jpg "Western Blot: Laminin beta 3 Antibody [NBP3-43799]")
Western Blot: Laminin beta 3 Antibody [NBP3-43799]
Analysis in human lung tissue.Applications for Laminin beta 3 Antibody (CL3353) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Laminin beta 3
Alternate Names
BM600-125kDa, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa
Gene Symbol
LAMB3
Additional Laminin beta 3 Products
Product Documents for Laminin beta 3 Antibody (CL3353) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Laminin beta 3 Antibody (CL3353) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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