Laminin beta 3 Antibody (CL3353) - BSA Free

Novus Biologicals | Catalog # NBP2-46622

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Rabbit

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3353

Format

BSA Free
View all Laminin beta 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q13751, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 3 Antibody (CL3353) - BSA Free

Laminin beta 3 Antibody (CL3353) - BSA Free Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Analysis in human lung tissue.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Analysis in human skin and pancreas tissues. RNA-seq data are presented for the same tissues.
Laminin beta 3 Antibody (CL3353) - BSA Free Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of A-431 cells showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human fallopian tube shows moderate to strong positivity in basement membrane of glandular cells.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human pancreas shows no positivity in glandular cells as expected.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human prostate shows moderate to strong positivity in basement membrane of glandular cells.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of human skin shows moderate positivity in the basement membrane of the basal layer of epidermal cells.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of rat kidney shows strong positivity in basement membrane in cells in glomeruli.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of rat lung shows moderate to strong positivity in basement membrane of pneumocytes.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of rat rectum shows moderate positivity in basement membrane of glandular cells, as well as strong positivity in endothelial cells.
Laminin beta 3 Antibody (CL3353) - BSA Free Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Immunohistochemistry: Laminin beta 3 Antibody (CL3353) [NBP3-52469]

Staining of rat skin shows moderate to strong positivity in basement membrane basal epidermal cells.

Applications for Laminin beta 3 Antibody (CL3353) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-46622 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin beta 3

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Alternate Names

BM600-125kDa, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa

Entrez Gene IDs

3914 (Human)

Gene Symbol

LAMB3

UniProt

Q13751 (Human)

Additional Laminin beta 3 Products

Product Documents for Laminin beta 3 Antibody (CL3353) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Laminin beta 3 Antibody (CL3353) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Laminin beta 3 Antibody (CL3353) - BSA Free

Customer Reviews for Laminin beta 3 Antibody (CL3353) - BSA Free (1)

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  • Laminin beta 3 Antibody (3353)
    Name: Apu Saikia
    Application: Immunocytochemistry
    Sample Tested: Rabbit tissue
    Species: Rabbit
    Verified Customer | Posted 05/04/2018
    1 hour incubation at 1:500 dilution worked very well for immunohistochemistry

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