LANA2 Antibody (CM-A807) [DyLight 405]

Novus Biologicals | Catalog # NB200-167V

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Virus

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CM-A807
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Product Specifications

Immunogen

Recombinant LANA2 fusion protein.

Reactivity Notes

Reacts with viral LANA2 protein and shows no cross-reactivity to cellular proteins. Also reacts with human and mouse viral KSHV proteins.

Localization

Nuclear

Specificity

This is specific for KSHV LANA2 (residues 143-310 epitope recognition).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for LANA2 Antibody (CM-A807) [DyLight 405]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: LANA2

LANA2 (latency-associated nuclear antigen 2; also known as viral interferon regulatory factor 3) is a Kaposi's sarcoma-associated herpesvirus (KSHV) latent protein which is encoded by ORFK10.5 and is exclusively expressed by virus infected B- cells. LANA2 has been shown to be critical in KSHV-mediated tumorigenesis as it inhibits p53 function, dsRNA-activated protein kinase R (PKR)-dependent apoptosis, nuclear factor kB activity, interferon regulatory factor 7-mediated interferon signal transduction and virus-mediated transcriptional activation of the alpha-interferon promoter. LANA2 stabilizes and triggers the induction of HIF-1alpha's transcriptional activity and disrupts PODs (PML oncogenic domains). Moreover, LANA2 has been shown to be required for the survival of primary effusion lymphoma (PEL) cells infected with KSHV alone or dually infected by Epstein-Barr virus (EBV) and KSHV, wherein it shows nuclear and cytoplasmic expression.

Alternate Names

LANA 2, LANA-2, Latency-associated nuclear antigen 2, vIRF 3, vIRF3, vIRF-3

Additional LANA2 Products

Product Documents for LANA2 Antibody (CM-A807) [DyLight 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LANA2 Antibody (CM-A807) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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