Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Chinese Hamster

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 310F7.02

Format

Azide and BSA Free
View all Langerin/CD207 Primary Antibodies »

Product Specifications

Immunogen

Langerin/CD207 was used as immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Cryosections of human skin, 1:200 dilution, staining for 1 hour at 37 degrees. Dapi nuclear stain used to define architecture. Langerin staining is visible in the epidermis. Image submitted from verified customer review.
Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Staining of human skin paraffin section with 310F7.02
Flow Cytometry: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Flow Cytometry: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Flow Cytometry: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Saining with 310F7.02 o f cells derived from overnight culture o f porcine skin biopsies permeabilized (B) or not(A).
Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Staining of CHO cells transfected (right) or not (left) with mouse Langerin.
Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Porcine skin cryosections stained with Langerin antibody
Flow (Cell Surface): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Flow (Cell Surface): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Flow (Cell Surface): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Analysis using the Allophycocyanin conjugate of DDX0361P-100. Staining of Langerin in stably transfected CHO using Langerin antibody at 1ug/ml. Blue histogram represents isotype control (1ug/ml), red histogram is anti-Langerin. Total viable cells were use
Flow (Intracellular): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Flow (Intracellular): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100]

Flow (Intracellular): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Analysis using the PE conjugate of DDX0361P-100. Staining of Langerin in stably transfected CHO cells (A) or DC-SIGN in stably transfected HeLa cells (negative control) (B) using antibody at 1ug/ml. Blue histogram in both panels represents isotype control.

Applications for Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Reviewed Applications

Read 1 review rated 5 using DDX0361P-100 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

Tris-NaCl (pH 8.0)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Langerin/CD207

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Alternate Names

CD207

Gene Symbol

CD207

Additional Langerin/CD207 Products

Product Documents for Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free

Customer Reviews for Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free (1)

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  • Langerin/CD207 Antibody (310F7.02)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: human skin cryosections
    Species: Human
    Verified Customer | Posted 04/03/2017
    This antibody worked great for IF on cryosections of human skin at a 1:200 dilution, staining for 1 hour at 37 degrees. Dapi nuclear stain was used to define architecture. Langerin staining is visible in the epidermis.
    Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free DDX0361P-100

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail