LARP1 Antibody

Novus Biologicals | Catalog # NBP1-19128

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all LARP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human La ribonucleoprotein domain family, member 1 using the numbering given in entry NP_291029.2 (GeneID 23367).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LARP1 Antibody

Western Blot: LARP1 Antibody [NBP1-19128]

Western Blot: LARP1 Antibody [NBP1-19128]

LARP1-Antibody-Western-Blot-NBP1-19128-img0017.jpg
Immunoprecipitation: LARP1 Antibody [NBP1-19128]

Immunoprecipitation: LARP1 Antibody [NBP1-19128]

Immunoprecipitation: LARP1 Antibody [NBP1-19128] - Detection of human LARP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 NBP1-19128 used for IP at 3 ug per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody NBP1-19129. For blotting immunoprecipitated LARP1, NBP1-19128 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
LARP1 Antibody

Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] -

Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] - Section of human colon carcinoma. Antibody:Affinity purified rabbit anti-LARP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
LARP1 Antibody

Western Blot: LARP1 Antibody [NBP1-19128] -

Western Blot: LARP1 Antibody [NBP1-19128] -Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, HepG2, and MCF-7 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-LARP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for LARP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID 25951186)

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LARP1

LARP1 (la-related protein 1) is part of a family of proteins that bears a La motif. The La motif was first identified in the La protein which was isolated as an autoreactive antigen in patients afflicted with systemic lupus erythematosus. Like the La protein, LARP proteins appear to be RNA-binding proteins, and are proposed to play a role in translation elongation and termination. Alternative names for LARP1 include la ribonucleoprotein domain family member 1, LARP, and KIAA0731.

Alternate Names

KIAA0731LARPMGC19556, La ribonucleoprotein domain family member 1, La ribonucleoprotein domain family, member 1, la-related protein 1

Entrez Gene IDs

23367 (Human)

Gene Symbol

LARP1

UniProt

Q6PKG0 (Human)

Additional LARP1 Products

Product Documents for LARP1 Antibody

Certificate of Analysis

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Product Specific Notices for LARP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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