LAT [p Tyr191] Antibody (2E4) - BSA Free

Novus Biologicals | Catalog # NBP3-15162

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2E4 expressed in HEK293

Format

BSA Free
View all LAT Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-LAT (Y191). UniProt ID (O43561).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LAT [p Tyr191] Antibody (2E4) - BSA Free

Western Blot: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate (treated with EGF or Pervanadate). All lanes: LAT antibody at 2.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa.
Immunocytochemistry/Immunofluorescence: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Immunofluorescence staining of Hela cells with at LAT antibody 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - IHC image of LAT antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - IHC image of LAT antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitation: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Immunoprecipitating LAT in A549 whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of LAT antibody in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: LAT antibody (3ug) + A549 whole cell lysate (1mg). Lane 3: A549 whole cell lysate (20ug).

Applications for LAT [p Tyr191] Antibody (2E4) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: LAT

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Gene Symbol

LAT

Additional LAT Products

Product Documents for LAT [p Tyr191] Antibody (2E4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LAT [p Tyr191] Antibody (2E4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LAT [p Tyr191] Antibody (2E4) - BSA Free

There are currently no reviews for this product. Be the first to review LAT [p Tyr191] Antibody (2E4) - BSA Free and earn rewards!

Have you used LAT [p Tyr191] Antibody (2E4) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies